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KMID : 0613820130230010069
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Journal of Life Science
2013 Volume.23 No. 1 p.69 ~ p.78
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Antiadipogenic Effect of Vitis amurensis Root Methanol Extract and Its Solvent Fractions in 3T3-L1 Preadipocytes
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Park Jung-Ae
Jin Kyong-Suk
Oh You-Na
Hyun Sook-Kyung
Choi Yung-Hyun
Kwon Hyun-Ju
Kim Byung-Woo
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Abstract
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Vitis amurensis Rupreche, a sort of grape, grows naturally in Asian countries. It is known for important biological effects such as antioxidation, anti-inflammation, neuroprotection, and angiogenesis inhibition. Although its root is used as a traditional folk medicine in Korea, the root's biological activities are poorly studied. In the present study, the effects of V. amurensis root methanol extract (VARM) and its solvent fractions on adipocyte differentiation and adipogenesis in 3T3-L1 preadipocytes were investigated. The VARM significantly suppressed adipocyte differentiation, lipid accumulation, and the triglyceride (TG) content of 3T3-L1 preadipocytes in a dose-dependent manner, without cytotoxicity. To identify active molecules, the VARM was fractionated with a series of organic solvents including dichloromethane (CH₂CI₂), ethyl acetate (EtOAc), and n-butanol (n-BuOH). All the fractions also showed inhibition of lipid accumulation in the 3T3-L1 preadipocytes. The CH₂CI₂ fraction showed the most powerful anti-obesity effect through the modulation of cytidine-cytidine-adenosine-adenosinethymidine (CCAAT)/enhancer binding protein α (C/EBPα), C/EBPβ, peroxisome proliferator-activated receptor r (PPARr) gene and protein expression. Oleanolic acid was one of the main active compounds involved in the anti-obesity activity of the V. amurensis root. These results provide important new insight into the potential potent anti-adipogenic effect of the V. amurensis root and illustrate that one of the main compounds involved in this effect is oleanolic acid.
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KEYWORD
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Vitis amurensis Rupreche root, 3T3-L1 preadipocyte, anti-adipogenic effect, oleanolic acid
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